Appendix P-I-A. The principal purpose of plant containment is to avoid the unintentional transmission of a recombinant DNA-containing plant genome, including nuclear or organelle hereditary material or release of recombinant DNA-derived organisms associated with plants.
Appendix P-I-B. The containment principles are based on the recognition that the organisms that are used pose no health threat to humans or higher animals (unless deliberately modified for that purpose), and that the containment conditions minimize the possibility of an unanticipated deleterious effect on organisms and ecosystems outside of the experimental facility, e.g., the inadvertent spread of a serious pathogen from a greenhouse to a local agricultural crop or the unintentional introduction and establishment of an organism in a new ecosystem.
Appendix P-I-C. Four biosafety levels, referred to as Biosafety Level (BL) 1 - Plants (P), BL2-P, BL3-P, and BL4-P, are established in Appendix P-II, Physical Containment Levels. The selection of containment levels required for research involving recombinant DNA molecules in plants or associated with plants is specified in Appendix P-III, Biological Containment Practices. These biosafety levels are described in Appendix P-II, Physical Containment Levels. This appendix describes greenhouse practices and special greenhouse facilities for physical containment.
Appendix P-I-D. BL1-P through BL4-P are designed to provide differential levels of biosafety for plants in the absence or presence of other experimental organisms that contain recombinant DNA. These biosafety levels, in conjunction with biological containment conditions described in Appendix P-III, Biological Containment Practices, provide flexible approaches to ensure the safe conduct of research.
Appendix P-I-E. For experiments in which plants are grown at the BL1 through BL4 laboratory settings, containment practices shall be followed as described in Appendix G, Physical Containment. These containment practices include the use of plant tissue culture rooms, growth chambers within laboratory facilities, or experiments performed on open benches. Additional biological containment practices should be added by the Greenhouse Director or Institutional Biosafety Committee as necessary (see Appendix P-III, Biological Containment Practices), if botanical reproductive structures are produced that have the potential of being released.