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Plasmid Collections

We are pleased to stock AAV- and LV-optogenetic constructs from Dr. Karl Deisseroth at Stanford University. For first time order of these vectors you will have to fill an MTA form with Dr. Deisseroth. To obtain MTA, please contact Dr. Karl Deisseroth and Charu Ramakrishnan. Please upload a copy of your MTA when placing your online order.
Contact email of Charu Ramakrishnan: charur@stanford.edu.  
Contact email of Dr. Karl Deisseroth: deissero@stanford.edu

AAV constructs

AAV-EF1a-DIO-EYFP
AAV-EF1a-DIO-mCherry
AAV-EF1a-DIO-hCHR2(H134R)-mCherry
AAV-EF1a-DIO-hCHR2(H134R)-EYFP
AAV-EF1a-DIO-eNpHR 3.0-EYFP
AAV-EF1a-DIO-chETA-EYFP
AAV-CaMKIIa- EYFP
AAV-CaMKIIa- mCherry
AAV-CaMKIIa- hCHR2(H134R)-EYFP
AAV-CaMKIIa- hCHR2(H134R)-mCherry
AAV-CaMKIIa- eNpHR 3.0-EYFP
AAV-hSyn- hCHR2(H134R)-EYFP
AAV-hSyn- hCHR2(H134R)-mCherry
AAV-hSyn- eNpHR 3.0-EYFP
AAV-CaMKIIa- C1V1 (t/t)-TS- EYFP
AAV-CaMKIIa- C1V1 (t/t)-TS- mCherry
AAV-EF1a-DIO- C1V1 (t/t)-TS- EYFP
AAV-EF1a-DIO- C1V1 (t/t)-TS- mCherry
AAV-CaMKIIa- hCHR2(C128S/D156A)-EYFP
AAV-CaMKIIa- hCHR2(C128S/D156A)-mCherry
AAV-EF1a-DIO- hCHR2(C128S/D156A)-EYFP
AAV-EF1a-DIO- hCHR2(C128S/D156A)-mCherry
AAV- CaMKIIa - hCHR2(et/tc)-EYFP
AAV- CaMKIIa - hCHR2(et/tc)-mCherry
AAV-CaMKIIa- hCHR2(E123A)-EYFP
AAV-CaMKIIa- hCHR2(E123A)-mCherry
AAV-EF1a-DIO- hCHR2(et/tc)-EYFP
AAV-EF1a-DIO- hCHR2(et/tc)-mCherry
AAV-EF1a-DIO- hCHR2(E123A)-EYFP
AAV-EF1a-DIO- hCHR2(E123A)-mCherry
AAV-EF1a-DIO- eNpHR 3.0- mCherry
AAV- CaMKIIa- eNpHR 3.0- mCherry
AAV- hSyn- eNpHR 3.0- mCherry
AAV- hSyn- hCHR2(E123A)-EYFP
AAV- hSyn- EYFP
AAV-EF1a-mCherry-IRES-WGA-Cre
AAV-GFAP-DIO-hCHR2(H134R)-EYFP
AAV-GFAP-DIO-hCHR2(H134R)-mCherry
AAV- CaMKIIa- eArch3.0- EYFP
AAV-EF1a-DIO- eArch3.0- EYFP
AAV- CaMKIIa- eArchT3.0- EYFP
AAV- hSyn- mCherry
AAV-EF1a-DIO- hCHR2(T159C)- EYFP
AAV-EF1a-DIO- hCHR2(T159C)- mCherry
AAV- hSyn- eArch3.0- EYFP
AAV- hThy-1- eNpHR 3.0- EYFP
AAV- CaMKIIa- C1V1 (E162T)- p2A- EYFP
AAV-CaMKIIa- hCHR2(T159C)- p2A- EYFP
AAV-CaMKIIa- hCHR2(T159C)- p2A- mCherry
AAV-CaMKIIa- hCHR2(et/tc)- p2A- EYFP
AAV-CaMKIIa- hCHR2(et/tc)- p2A- mCherry
AAV-CaMKIIa- C1V1 (E162T)- TS-p2A- EYFP
AAV-CaMKIIa- C1V1 (E162T)- TS-p2A- mCherry
AAV-CaMKIIa- C1V1 (t/t)- TS-p2A- EYFP
AAV-CaMKIIa- C1V1 (t/t)- TS-p2A- mCherry
AAV-EF1a-DIO- hCHR2(et/tc)- p2A- EYFP
AAV-EF1a-DIO- hCHR2(et/tc)- p2A- mCherry
AAV-EF1a-DIO- C1V1(E162T)- TS- p2A- EYFP
AAV-EF1a-DIO- C1V1(E162T)- TS- p2A- mCherry
AAV-EF1a-DIO- C1V1(t/t)- TS- p2A- EYFP
AAV-EF1a-DIO- C1V1(t/t)- TS- p2A- mCherry

Lentiviral constructs

pLenti- CaMKIIa- eArchT3.0- EYFP
pLenti- CaMKIIa- eArch3.0- EYFP
pLenti- CaMKIIa- eMac3.0- EYFP
pLenti- CaMKIIa- C1V1(E122T)-TS- EYFP
pLenti- CaMKIIa- C1V1(E162T)-TS- EYFP
pLenti- CaMKIIa- C1V1(E122T/E162T)-TS- EYFP
pLenti- CaMKIIa- hCHR2(C128S/D156A)- EYFP
pLenti- CaMKIIa- hCHR2(T159C)- EYFP-WPRE
pLenti- CaMKIIa- hCHR2(E123T/T159C)- EYFP-WPRE
pLenti- EF1a - hCHR2- EYFP-WPRE (a.k.a. pLECYT)
pLenti- CaMKIIa- hCHR2-mCherry-WPRE
pLenti- CaMKIIa- hCHR2- EYFP -WPRE
pLenti- CaMKIIa- hCHR2(H134R)- EYFP -WPRE
pLenti- hSyn - hCHR2(H134R)- EYFP –WPRE
pLenti- CaMKIIa- eNpHR 3.0- EYFP
pLenti- hSyn- eNpHR 3.0- EYFP

We are pleased to stock LV-optogenetic constructs from Dr. Ed Boyden at MIT. No MTA required for ordering these plasmids.

Lentiviral constructs

FCK-Halo-GFP
FCK-ChR2-GFP
FCK-Arch-GFP
FCK-Mac-GFP
FCK-ArchT-GFP

Please note that the FCK and the CaMKIIa promoters are the same

Plasmids developed in our laboratory

We also offer the following plasmids developed in our laboratory.

pLenti-mPGK-LacZ
pLenti-mPGK-EGFP
pLenti-mPGK-mCherry
pLenti-mPGK-Luc-IRES-EGFP
pLenti-mPGK-AcGFP
pLenti-hCMV-EGFP
pLenti-hCMV-mCherry
pLenti-mPGK-LacZ
pLenti-hSyn- EGFP
pLenti-hSyn- mCherry
pLenti-hSyn- mCherry-IRES-EGFP
pLenti-hSyn- Luc-PGK-GFP
pLenti-FCK- EGFP
pLenti-hGFAP- EGFP

 

 

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