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Services and Fees

Lentiviral Vectors
Prep  Vector
titer*
Volume
Provided
Prep Concentration Gold
Titering*
RCR
assay*
USC External Academic Corporate
Small  1x10E7
vg/mL
10 ML  -  +  - $200 $250 $300
Medium   1x10E9
vg/mL
20 ML  +  +  + $600 $720 $850
Large  1X10E9
vg/mL
100 ML  +  +  + $1,750 $2,100 $2,500

Vector titer*-guaranteed minimal; usually higher

Gold Titering*-titers will be measured by three different assays: P24 ELISA, real-time PCR, targeted cells counting if reporter transgene*

RCR assay *- mandatory for the concentrated preps and optional for the non-concentrated (small) 
prep. Extra charge of $50 applied

Approximate turnaround time is two weeks for the non-concentrated vectors and four to six weeks for the concentrated vectors

Adeno-Associated Vectors
Prep  Vector
titer*
Volume
Provided
Prep Concentration Gold
Titering*
USC External AcademicC Corporate
Prep     Vector
titer*
Volume
Provided
Prep Concentration Gold Titering* USC External Academic Corporate
 Small 1x10E11
vg/mL
100 µL + + $200 $250 $300
 Standard 1x10E12
vg/ml
1 mL + + $1750 $2100 $2500
 Megta 1X10E13
vg/mL
2.5 mL + + $7500 $10,000 $12,500

Vector titer*-guaranteed minimal; usually higher

Gold Titering*-titers will be measured by two different assays: real-time PCR, targeted cells counting if reporter transgene included

Approximate turnaround time is two to three weeks for the Standard Prep and three-to four weeks for the Mega Prep

Herpes Simplex Virus

Recombinant HSV1
Transfection of linearized shuttle plasmid and viral DNA; purification through >3 rounds of limiting dilution, expansion culture for subsequent production. Concentrated HSV stock 500 μL of 107 pfu/μL. Cost*

High concentration stock produced from existing expansion culture Cost** 

  USC External Academic Corporate
$3,000 $3,500 $4,200
**  $1,000 $1,250 $1,500

 

Other Services
Service   Description Cost
Cloning Subcloning of cDNA into vectior cassette $850
Cloning Single-step cloning -- additional plasmid $600
Cloning Multiple step/large fragments (>10kb)/insert by
PCR/Genetic mutagenesis
$1,250
Plasmid Prep Plasmid amplification-Maxi Prep $200
p24 ELISA p24 ELISA/Plate $500
Comp cells HB101 1ml (20x50ul)>108cfu/µg $150
Cesium Prep Cesium Grade DNA 1mg $750