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College of Pharmacy

Tray of COVID-19 Saliva Test Tubes

Diagnostic Genomics Lab

SalivirDetect

SalivirDetect is a Quantitative Reverse Transcription Polymerase Chain Reaction method applied to crude saliva samples, with no need for RNA isolation. 

About SalivirDetect

SalivirDetect is a Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) method applied to crude saliva samples, with no need for Ribonucleic Acid (RNA) isolation. Small volumes of saliva are needed for each test (20ul), however larger volumes of saliva (5ml) enable automation by robotic liquid handlers.

SalivirDetect can be broadly implemented as it does not require saliva collection tubes with preservatives and does not require specialized equipment for RNA extraction. The simplicity and flexibility of SalivirDetect means that it is less likely to be affected by supply chain bottlenecks.

We wish to recognize and credit Emergency Use Authorization 202097  - SalivaDirect, Nathan Grubaugh, Ph.D. at Yale School of Public Health - as our assay is similar to their methodology with some modifications.


 


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