The Flow Cytometry Core, one of three core facilities supported by the COBRE Center for Targeted Therapeutics, is a facility that provides access to state-of-the-art resources, training and services in flow cytometry for University of South Carolina researchers, collaborators and external organizations.
We are housed in the Coker Life Sciences Building and serve a large community of researchers studying a wide range of topics in drug discovery and biomedical sciences.
Flow Cytometry Core Resources
The BD LSR II has digital electronics for increased speed, a 3-laser optical bench, and can detect 8 fluorescent parameters plus two physical parameters and a time parameter.
- Coherent Sapphire solid state 488 nm
- JDS Uniphase 1344P HeNe 640 nm
- Coherent Vioflame solid state 405 nm
The BD FACS Aria is a high-speed cell sorter. New-generation digital electronics allow high speed analysis and sorting (>20,000 cells per second).
The FACS Aria optical system is configured with three excitation lasers and can detect 9 fluorescent parameters simultaneously (plus 2 physical parameters and a time parameter).
Additionally, the FACS Aria offers numerous sorting options which include 4-way sorting, sorting to microtiter plates or glass slides, and customizable settings to optimize sort yield and purity.
The FACS Aria laser excitation wavelengths and partial list fluorochromes that can be utilized on the instrument are listed in the table.
The SH-800 uses disposable microfluidics chips for easily switching between sample sources.
- Simplified user interface
- 405, 488, 561 and 638nm excitation lasers
- Compatible with tube of 96 well plate deposition