How our brain develops and operates has captivated the mind of scientists for centuries. During development, neurons extend highly dynamic axons that interpret guidance cues in their environment to navigate towards their final synaptic targets. Axons often extend great distances from the soma and adopt distinct behaviors to ensure the accuracy of circuit wiring. Recent advances in imaging technologies, including light-sheet fluorescent microscopy and super-resolution microscopy, have provided insights into axonal development in unparalleled detail, enabling the reconstruction of neural circuits in vivo and the discovery of the cellular and molecular mechanisms governing circuit development. In their review article, Cory Weaver and Fabienne Poulain describe how improved microscopy has revolutionized our understanding of axonal development. They notably discuss how light-sheet and super-resolution microscopy led to new discoveries at the cellular and subcellular scales by imaging  circuit wiring and the subcellular dynamics driving axon  guidance with extreme precision. Great analysis!