Faculty and Staff
Chang-uk Lim, Ph.D.
|Title:||Research Assistant Professor
Director, Flow Cytometry Core
|Department:||Drug Discovery & Biomedical Sciences (DDBS)
College of Pharmacy
College of Pharmacy
715 Sumter Street - CLS 714
Columbia, SC 29208
Ph.D. Cell and Molecular Biology, Colorado State University, 2004
M.S. Environmental and Radiological Health Sciences, Colorado State University, 1998
B.S. Biology, Korea University, 1995
Chang-uk Lim, Ph.D., joined UofSC as a Research Assistant Professor and the Flow Cytometry Core Director in May 2011. Lim has more than 18 years of experience in cell sorting, cell cycle analysis, apoptosis and immunophenotyping. Specific areas of interest include understanding the mechanisms, which allow cancer cells to develop resistance to chemotherapy at the cellular and molecular levels. His research focuses on functions of several key signaling molecules during development and progression of human diseases, particularly in breast cancer and leukemia.
Lim runs the COBRE Flow Cytometry Core, which provides the following services:
- Detection of cell-surface and intracellular antigens
- Cell cycle, cell division and DNA ploidy analysis
- Apoptosis assays
- DNA repair assays
- High-throughput flow cytometric assays
- Side population and CD marker assays for stem cells
- Direct cell cloning
- Aseptic sorting of living cell populations
- Development and trouble-shooting of new flow cytometric assays
Porter DC, Farmaki E, Altilia S, Schools GP, West DK, Chen M, Chang BD, Puzyrev AT, Lim CU, Rokow-Kittell R, Friedhoff LT, Papavassiliou AG, Kalurupalle S, Hurteau G, Shi J, Baran PS, Gyorffy B, Wentland MP, Broude EV, Kiaris H and Roninson IR: Cyclin-dependent kinase 8 mediates chemotherapy-induced tumor-promoting paracrine activities. Proc. Natl. Acad. Sci. USA. 2012 Aug 21; 109(34): 13799-13804. doi: 10.1073/pnas.1206906109. Epub 2012 Aug 6.
Levina E, Ji H, Chen M, Baig MR, Oliver D, Ohouo P, Lim CU, Schools GP, Carmack CS, Ding Y, Broude EV, Roninson IB, Buttyan R and Shtutman MS: Identification of novel genes that regulate androgen receptor signaling and growth of androgen-deprived prostate cancer cells. Oncotarget 2015 May 30; 6(15): 13088-104
McDermott MS, Chumanevich AA, Lim CU, Liang J, Chen M, Altilia S, Oliver D, Rae JM, Shtutman M, Kiaris H, Győrffy B, Roninson IB and Broude EV: Inhibition of CDK8 mediator kinase suppresses estrogen dependent transcription and the growth of estrogen receptor positive breast cancer. Oncotarget 2017 Feb 21; 8(8): 12558-12575. doi: 10.18632/oncotarget.14894.
Chen M, Liang J, Ji H, Yang Z, Altilia S, Hu B, Schronce A, McDermott MS, Schools GP, Lim CU, Oliver D, Shtutman MS, Lu T, Stark GR, Porter DC, Broude EV and Roninson IB: CDK8/19 mediator kinases potentiate induction of transcription by NFkB. Proc Natl Acad Sci U S A. 2017 Sep 19;114(38):10208-10213. doi: 10.1073/pnas.1710467114. Epub 2017 Aug 30.