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Leica AS MDW Live cell imaging system
-Xe monochromator for narrow excitation band -Motorized stage for multiple x,y positions per time point -Specimen chamber: temperature, 5% CO2 atmosphere
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2 Leica DM IRE
- environmental chamber - 10x to 100x - DAPI (blue) , FITC (gree), TRITC (red), and Cy5 (far red) filter sets
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3 Carl Zeiss Axiovert 200s
-Cell migration -Proliferation -Apoptosis -Mitosis -Cell lineage tracking
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Olympus IX81
- heated stage insert- filter wheel for short-term live cell imaging of rapid processes
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Carl Zeiss LSM700 confocal microscope
- Confocal point scanning - 3D reconstruction - Multi-field time lapse - Ratiometric concentration determination - Tile and stitch - FRET (Förster resonance energy transfer) - FRAP (fluorescence recovery after photobleaching)
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ZEISS PALM system for laser microdissection
PALM MicroBeam uses a focused laser beam to cut out and isolate the selected specimen without contact. The patented laser catapult isolates the target area fast and uncontaminated. It allows to obtain the homogenous analysis material necessary for meaningful scientific results. The short laser pulse minimizes time heat transfer to adjacent areas, allowing isolation of vital life cells. Because analyses of gene expression patterns rely on exactly-separated analytical material, unwanted cells may alter your results and conceal the signals of the relevant cells. PALM MicroBeam prevents this by allowing you to define cells and tissue regions precisely, ensuring your results are exact and reproducible. Applicable to cryosections, FFPE (formalin-fixed and paraffin embedded) tissue, native tissue like fresh plants, live cells and chromosomes - without contamination. Suitability for laser microdissection and analysis for DNA, RNA and protein isolation - whether from archive material or live cells Works with standard glass slides